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KMID : 0385520040170020192
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Analytical Science & Technology
2004 Volume.17 No. 2 p.192 ~ p.197
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Development of the Discrimination Methods for Geographical Origin of Bracken(Pteridium aquilinum) by Capillary Electrophoresis
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김은영/Kim EY
김정현/정경숙/류미라/Kim JH/Chung KS/Rhyu MR
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Abstract
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The discrimination of bracken (Korean vs. Chinese) has been attempted using capillary electrophoresis (CE). Bracken (Pteridium aquilinum) was extracted with 30% methanol and separated on a uncoated fused-silica (50 ㎛ × 27 ㎝) capillary. Conditions for optimal analysis include: temperature, 40 ℃; voltage, 8 ㎸; and pressure injection time, 5 sec. The optimal separation buffer was 0.3 M borate buffer (pH 8.5) containing 40 mM CHAPS with 30% ethylene glycol. Under the optimal conditions established for CE, the ratio of specific peak area (peak PA-1) to other peak area (peak PA-2) was effective in discrimination of Korean and Chinese bracken. The mean accuracy for discrimination of Korean and Chinese brackens were 80% and 86%, respectively.
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KEYWORD
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